熱甩尾儀根據(jù)D"amour/Smith原理和方法測(cè)量大鼠、小鼠尾巴部受紅外熱刺激時(shí)的痛覺(jué)閾值。

實(shí)驗(yàn)步驟及方法

· 該儀器使用可調(diào)強(qiáng)度的紅外光源紅外光通過(guò)一拋物鏡面反射聚焦在實(shí)驗(yàn)動(dòng)物的尾巴上；

· 操作人員將實(shí)驗(yàn)動(dòng)物置于儀器上把動(dòng)物的尾巴放在紅外光源處接受熱輻射刺激；

· 當(dāng)動(dòng)物感覺(jué)到疼痛時(shí)尾巴會(huì)左右甩動(dòng)或者輕敲臺(tái)面內(nèi)置傳感器會(huì)立刻檢測(cè)到尾巴的活動(dòng)停止計(jì)時(shí)和關(guān)閉光源；

· 儀器自動(dòng)記錄反應(yīng)時(shí)間和光源強(qiáng)；

· 數(shù)據(jù)可通過(guò)U盤或USB數(shù)據(jù)線導(dǎo)出到電腦；

· 該型號(hào)的設(shè)備在世界上使用和驗(yàn)證超過(guò)3000次積累了數(shù)十年的經(jīng)驗(yàn)；

型號(hào)37560

主要特點(diǎn)

· 尾部輕拍可自動(dòng)記錄或手動(dòng)評(píng)分；
· 自動(dòng)化程度高避免人為因素引起的誤差；
· 觸摸屏控制操控方便顯示直觀；
· 可獨(dú)立工作也可連接電腦使用；
· 測(cè)試平臺(tái)平整表面無(wú)突出和遮擋部件；
· 可選配紅外熱輻射校準(zhǔn)儀用于校準(zhǔn)紅外光源；
· 多種單位可轉(zhuǎn)換；

· 該儀器適合大鼠和小鼠的測(cè)試；

· 有大鼠、小鼠兩種不同的固定器可供選擇；

正在進(jìn)行大鼠甩尾測(cè)試

儀器的操作
· 主機(jī)內(nèi)包括紅外光源、傳感器、微控制器；
· 自動(dòng)檢測(cè)尾巴的活動(dòng)情況自動(dòng)計(jì)時(shí)延遲時(shí)間會(huì)被自動(dòng)記錄；
· 可選配傾斜的老鼠固定器將老鼠尾巴保持在 45 度向上的位置。

可選配的小鼠固定器

控制面板

· 數(shù)據(jù)格式通用化可使用其他軟件進(jìn)行統(tǒng)計(jì)；
· 帶儲(chǔ)存功能用于實(shí)驗(yàn)數(shù)據(jù)的保存；

· 可聯(lián)網(wǎng)通過(guò)遠(yuǎn)程網(wǎng)絡(luò)對(duì)實(shí)驗(yàn)方案進(jìn)行編輯；

校準(zhǔn)輻射計(jì)
· 用來(lái)對(duì)輻射強(qiáng)度進(jìn)行校準(zhǔn)；校準(zhǔn)輻射計(jì)貨號(hào)我37300；
· 確保兩個(gè)或多個(gè)裝置提供相同強(qiáng)度的熱傷害性刺激（輻射單位mW/cm2）
· 測(cè)量紅外光的能量的值（1s持續(xù)時(shí)間內(nèi)的1mW對(duì)應(yīng)1mJ）

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方法學(xué)文獻(xiàn)

F.E. D’mour & D.L. Smith"A Method for Determining Loss of Pain Sensation." J. Pharmacol. Exp. Therap. 7274-79, 1941.